Paul-Flechsig-Institut für Hirnforschung
 Universitätsmedizin Leipzig

Methods

Morphological Methods

  • classical histological staining techniques for nerve tissue
  • silver impragnation techniques (Golgi, Bielschowsky, Gallyas)
  • immunohistochemistry
  • enzymhistochemistry
  • quantitative morphology (videomicroscopy, image analysis, stereology)
  • electron microscopy (Zeiss 912 OMEGA)
  • laser capture microdissection (PALM)
  • live cell imaging
  • Kryocut and freezing microtom-techniques
  • parafin embedding
  • konfocal laser scanning microscopy (Zeiss LSM510 Meta)
  • multiphotonmikroscopy (Zeiss LSM510 NLO)
  • chromogene in situ hybridisation (CISH), fluoreszenz in sity hybridisation (FISH)
  • CLARITY

Neurochemical Methods

  • proteinbiochemical analytics
  • western-blotting
  • ELISA
  • enzymkinetics

Cell and Tissue Culture

  • cell lines, primary cell culture of neuron and gliacells
  • organotypical sclice cultures
  • knockdown experiments
  • fluoreszenz activated cell sorting (FACS)
  • patch clamp (in vitro)

Molecular biological Methods

  • basical techniques (cloning, PCR)
  • transient and permanent transfections
  • quantitative RT-PCR, northern-blotting
  • promotorstudies
  • southern-blotting
  • genotyping
  • EMSA
  • ChIRP
  • inducible neuronspecific genexpression
  • viral geneexpression

Behavioural Methods

  • Morris watermaze
  • 8-arm-radial maze
  • Rotarod
  • simulation of artificial hibernation
 
last update: 15.02.2017, 09:54 Uhr
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Paul-Flechsig-Institut für Hirnforschung